Gender Difference in the Composition of Fecal Flora in Laboratory Mice, as Detected by Denaturing Gradient Gel Electrophoresis (DGGE)

Application of denaturing gradient gel electrophoresis (DGGE) for assessing fecal pollution sources at a recreational beach.

We investigated the effectiveness of Escherichia coli community fingerprinting for identifying fecal pollution sources impacting a recreational beach. E. coli in water collected from the beach, nearby creek and storm sewer outfall were enumerated using membrane filtration, while E. coli communities were characterized following polymerase chain reaction analysis and denaturing gradient gel elect...

Denaturing gradient gel electrophoresis (DGGE) as a tool for identification of marine nematodes

Many phyla of marine invertebrates are difficult to identify using conventional morphological taxonomy. Larvae of a wider set of phyla are also difficult to identify as a result of conservation of morphology between species or because morphological characters are destroyed during sampling and preservation. DNA sequence analysis has the potential for identification of marine organisms to the spe...

Microbial Community Fingerprints by Differential Display Denaturing Gradient 1 Gel Electrophoresis ( DD - DGGE )

Denaturing Gradient Gel Electrophoresis (DGGE) in Microbial Ecology – Insights from Freshwaters

Although freshwater ecosystems support a great diversity of life, knowledge of its total diversity is far to be complete, particularly among microbes (Dudgeon et al., 2006). Aquatic microorganisms are pivotal in several biogeochemical processes, playing a key role in the recycling of organic material, or contributing to energy transfer in food webs, since they constitute food sources to micro-f...

Polymorphism in the APNH gene, detected by denaturing gradient gel electrophoresis.

Description: A 376 bp genomic DNA fragment corresponding to nucleotides 2 5 — 351 of the published cDNA coding sequence for the Na + /H+ antiport gene, APNH (1), was amplified by PCR using 5'-CGAGGCTGGCTCTGGAAGCAGCACCATG-3' and 5'-TATCTTCATGAGGCAGGCCAGAAGGAT-3' as the 5' and 3' primers respectively. PCR was performed with a programmable thermal cycler (MJ Research Inc.) using the following cond...